Multiplex genome engineering in eukaryotes
نویسندگان
چکیده
منابع مشابه
Precise Editing at DNA Replication Forks Enables Multiplex Genome Engineering in Eukaryotes.
We describe a multiplex genome engineering technology in Saccharomyces cerevisiae based on annealing synthetic oligonucleotides at the lagging strand of DNA replication. The mechanism is independent of Rad51-directed homologous recombination and avoids the creation of double-strand DNA breaks, enabling precise chromosome modifications at single base-pair resolution with an efficiency of >40%, w...
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ژورنال
عنوان ژورنال: Nature Reviews Genetics
سال: 2017
ISSN: 1471-0056,1471-0064
DOI: 10.1038/nrg.2017.103